Lyme Disease

by Dawn Burke, DVM

Lyme disease is caused by the spirochete (spiral shaped bacteria) Borrelia burgdorferi which is transmitted to dogs from the bite of an infected deer tick (Ixodes scapularis). The first case of Lyme disease in dogs was reported in 1984. Besides causing arthritis, Lyme disease can also
affect the kidneys, heart, and nervous system.

Ixodes ticks feed on three different hosts during their development from eggs to adults. When the eggs hatch, the larvae feed on small mammals such as rodents, which may
be carriers of the Lyme organism. In regions where winters are cold, it takes two years for ticks to mature, which is ideal for maintenance of infection. Tick larvae infected by feeding on rodents in the fall molt to nymphs the next spring, infecting the next generation of rodents and continuing the cycle. Ixodes ticks can produce up to 22,000 eggs in one egg clutch and may lay several clutches before they die. The tickʼs saliva has the ability to suppress the immune response of the host, allowing repeat feedings on the same host by many ticks.

Borrelia burgdorferi is transmitted to a host via tick saliva after a lag phase of 24 to 48 hours following attachment. In mammals, the spirochete takes up residence in the layer of cells that line the inside of blood vessels and may lie dormant in connective tissue. The spirochete is difficult to detect in blood or tissue and can evade both the hostʼs immune response and antibiotic therapy. It takes three to six weeks after infection for the production of antibodies by
the host. The incubation period for the disease in naturally infected dogs is not known, but in laboratory dogs clinical signs develop from two to five months after exposure to infected ticks.

The most common sign of Lyme disease in dogs is sudden onset of lameness, sometimes with fever, loss of appetite, and depression. One or more joints may be warm and swollen. The kidneys can also be affected, resulting in protein loss in the urine and kidney failure. Heart disease and neurological signs such as seizures can be seen in rare cases. It is felt that approximately 15-25% of dogs treated for Lyme arthritis will
become chronically infected and have incomplete resolution of signs or reoccurring disease.

One of the most confusing issues in the diagnosis of Lyme disease has been the significance of testing. A test result showing antibodies against Borrelia burgdorferi is merely an indication of exposure to the spirochete and by itself does not confirm a diagnosis of Lyme disease.

The tests most often used are the Snap 3DX (tests for Lyme, heartworm, and ehrlichiosis) and the Snap 4DX (tests for anaplasmosis in addition to the other three) made by Idexx Labs. These tests can be run in the veterinary hospital and do not need to be sent to a reference laboratory. The tests detect antibodies produced when live Borrelia burgdorferi interact with cells in the spleen of infected animals. Because of this, the test will be positive only if antibodies are present due to natural exposure and antibodies formed by vaccination for Lyme disease will not cause a positive test. This information was previously available only through the Western Blot test run by reference labs, which is time consuming and potentially difficult to interpret.

Recently, a Lyme Quantitative C6 antibody test has become available from Idexx Reference Lab. The test measures the amount of antibody present to the C6 antigen. Veterinarians can use this test to help decide whether a positive dog needs treatment and to help monitor the response to therapy of treated dogs.

Another test recommended for dogs that test Lyme positive is a urinalysis and a urine protein determination (microalbumin or protein:creatinine ratio). These tests help veterinarians determine whether Lyme nephritis, a fatal form of Lyme disease affecting the kidney, is present.

Despite all of the tests available, Lyme disease remains difficult to accurately diagnose since there is no test that will detect the presence of the spirochete itself. The tests available only detect the antibodies formed by the dogʼs immune system in response to exposure to the Borrelia
spirochete. Veterinarians must use their knowledge of Lyme disease and the tests available to determine which dogs need to be treated.

Practicing tick prevention is one way to help protect your dog from Lyme disease. Keeping dogs out of tall grass, woods, etc. can help. Ticks wait on tall grass and leaves and can detect a dogʼs (or personʼs!) presence by smelling carbon dioxide or other odorants produced by the dog.
When a dog (or other mammal) brushes against the vegetation where the tick is waiting, the tick transfers to the animal, grasping securely with claws on the end of its legs and finding a place to make a small incision and attach itself. Careful tick checks should be done every time a dog has been outside.

Many products are available that can be used to control ticks on dogs. Discuss with your veterinarian which products are best for your dogs. Due to the prevalence of tick-transmitted diseases in this area (Lyme, ehrlichiosis, anaplasmosis, Rocky Mountain Spotted Fever) it is recommended to practice tick control year round.

Vaccination is another way to help protect your dog from Lyme disease. Your veterinarian can help you decide if Lyme vaccine is indicated for your dog. The Lyme vaccine has been shown to be safe even when given to dogs that have already been infected with Lyme disease. The first year a dog is vaccinated, they receive 2 vaccinations spaced 2-3 weeks apart followed by annual revaccinations. Even when dogs are vaccinated for Lyme Disease, it is still recommended to use tick control not only to give added protection against Lyme Disease, but to help protect against other tick borne diseases.

Lyme disease can be a very serious health issue for your dog. Discuss with your veterinarian the best ways to help prevent your dog from getting this disease.